12z cell line  (Applied Biological Materials Inc)

Journal: Journal of Biological Physics

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells

doi: 10.1007/s10867-026-09712-1

Figure Lengend Snippet: Supplementary file2 3D rendering of a 12Z cell’s actin dynamics. 12Z cells were transfected with a LifeAct-GFP plasmid via nucleofection and imaged over time using lattice lightsheet microscopy. The movie depicts a 3D rendering of a 12Z cell (the same cell as in Movie Supplemental_2D.avi) exhibiting rapid protrusion dynamics. Total time elapsed = 1 hour (MP4 4077 KB)

Article Snippet: The 12Z cell line was purchased from Applied Biological Materials (Cat. #T0764).

Techniques:

Journal: Journal of Biological Physics

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells

doi: 10.1007/s10867-026-09712-1

Figure Lengend Snippet: Experimental setup. a 12Z cells were transfected with a LifeAct-GFP plasmid via nucleofection (an electroporation-based method) and imaged over time using volumetric lattice lightsheet microscopy, which employs long thin beams to illuminate the sample with subcellular resolution. b A maximum intensity projection showing that the boundary of the actin cytoskeleton (LifeAct-GFP) coincides with the plasma membrane. c An example output maximum intensity projection from a lattice lightsheet microscopy time-lapse of a cell transfected with LifeAct-GFP with frames from t = 0, 1 min, 5 min, 10 min, and 60 min. Scalebars = 20 µm

Article Snippet: The 12Z cell line was purchased from Applied Biological Materials (Cat. #T0764).

Techniques: Transfection, Plasmid Preparation, Electroporation, Microscopy, Clinical Proteomics, Membrane

Journal: Journal of Biological Physics

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells

doi: 10.1007/s10867-026-09712-1

Figure Lengend Snippet: 3D isosurfaces of 12Z cells obtained from lattice lightsheet imaging, with some notable cellular features ( a-d ) and representative time series ( e–g ). a Some cells exhibited actin waves at the leading edge of the cell and hair-like protrusions at the lagging edge of the cell (indicated by white arrows). Several cells also exhibited membrane ruffling ( b ), 3D filopodial extensions ( c , d ), and active protrusions ( d ). The dynamics of the membrane ruffling and lamellipodia growth were captured ( e ). Protrusion retraction ( f, g ) and formation ( f ) were also visualized. All scalebars = 20 µm

Article Snippet: The 12Z cell line was purchased from Applied Biological Materials (Cat. #T0764).

Techniques: Imaging, Membrane

Journal: Journal of Biological Physics

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells

doi: 10.1007/s10867-026-09712-1

Figure Lengend Snippet: Effect of E2 on 12Z shape and morphodynamics after 15 min (N = 7 cells per group) or 24 h (N = 5 cells per group) of treatment incubation. Circularity (a) , rate of change in circularity (b) , solidity (c) , and rate of change in solidity (d) over the duration of each time-lapse were extracted from binarized maximum intensity projections, and the means ± SD are plotted. * P ≤ 0.05, ** P ≤ 0.01

Article Snippet: The 12Z cell line was purchased from Applied Biological Materials (Cat. #T0764).

Techniques: Incubation

Journal: Journal of Biological Physics

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells

doi: 10.1007/s10867-026-09712-1

Figure Lengend Snippet: Impact of E2 on actin optical flow alignment in 12Z cells. a Optical flow (OF) was calculated from actin fluorescence for all timepoints to determine how the actin is moving within the video. The optical flow vectors are displayed on top of one of the corresponding actin fluorescence images. b The actin optical flow alignment (OF alignment) is displayed for the cell in ( a ). Higher values of optical flow alignment mean that the optical flow in that region is pointing in the same direction (see Materials and methods). Using the binarized masks from Fig. 5 the average optical flow alignment was found inside the protrusions and the cell body. The mean protrusion optical flow alignment (c) and mean ratio of protrusion optical flow alignment to cell body optical flow alignment ( d ) are plotted ± SD, representing individual cell data ( N = 5–7 cells per group). * P ≤ 0.05, ** P ≤ 0.01

Article Snippet: The 12Z cell line was purchased from Applied Biological Materials (Cat. #T0764).

Techniques: Fluorescence

Journal: Experimental and Therapeutic Medicine

Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death

doi: 10.3892/etm.2026.13080

Figure Lengend Snippet: Myrrh reduces the growth of human endometriotic cells by activating apoptotic signaling pathways. Endometriotic cells (12Z) and normal endometrial cells (T-HESC) were treated with the indicated concentrations of myrrh. (A) Cell viability was analyzed by measuring at 450 nm wavelength using MTT after 24 h. (B) To assess apoptotic or dead cell populations, 12Z cells were treated with increasing doses of myrrh for 24 h, and the percentages of Annexin V-positive and PI-positive cells were measured (Annexin V: Ex 494/Em 525 nm; PI: Ex 535/Em 617 nm). After 24 h of treatment with each concentration of myrrh, mitochondria-associated apoptotic proteins were analyzed by immunoblotting. (C) Representatives immunoblot images of Bax and Bcl-2 are shown. (D) A major monomeric Bax band at ~21 kDa (monomer Bax) and higher-molecular-weight bands corresponding to oligomeric forms (tetramer Bax, ~84 kDa) are indicated. (E) Representative immunoblot images of caspase-3, caspase-9 and PARP are shown. GAPDH was used as an internal control. (F) Densitometric analysis of protein levels. Data are expressed as relative intensity compared to control. Data are expressed as mean ± standard error of the mean. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01 and *** P<0.001. Ex, excitation; Em, emission; ; PARP, poly(ADP-ribose) polymerase.

Article Snippet: Immortalized human endometrial stromal T-HESCs were obtained from the American Type Culture Collection (#CRL-4003), and immortalized human endometriotic 12Z cells were purchased from Applied Biological Materials (#T0764).

Techniques: Protein-Protein interactions, Concentration Assay, Western Blot, Molecular Weight, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death

doi: 10.3892/etm.2026.13080

Figure Lengend Snippet: RNA sequencing analysis suggests a molecular mechanism underlying the anti-endometriosis effect of myrrh. 12Z cells were treated with myrrh (100 µg/ml) for 12 h. The RNA sequencing data sets provided by a commercial service were applied to GSEAs. (A) Hallmark and (B) KEGG analysis results are visualized by bubble plots; key pathways associated with endometriosis are marked in red. (C) GO analysis data are described by Cytoscape with enrichment map visualization; cutoff values are set at P>0.001 and q>0.5. (D) Representative enrichment plots of GSEAs upon myrrh treatment. GSEA, Gene set enrichment analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene Ontology.

Article Snippet: Immortalized human endometrial stromal T-HESCs were obtained from the American Type Culture Collection (#CRL-4003), and immortalized human endometriotic 12Z cells were purchased from Applied Biological Materials (#T0764).

Techniques: RNA Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death

doi: 10.3892/etm.2026.13080

Figure Lengend Snippet: Mechanism of action of myrrh in endometriosis. (A) 12Z were treated with the indicated concentrations of myrrh and TUDCA (200 µM). Cell viability was analyzed using MTT after 24 h, measured at a wavelength of 450 nm. (B) Schematic representation summarizing the experimental results and the potential mechanism by which myrrh exerts palliative effects on endometriosis. *** P<0.001. TUDCA, tauroursodeoxycholic acid; ATF6, activating transcription factor 6; IRE1α, inositol-requiring enzyme 1 alpha; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; GADD34, growth arrest and DNA damage-inducible protein 34; Bax, Bcl-2-associated X protein; Bcl2, B-cell lymphoma 2; Caspase 3, cysteine-aspartic acid protease 3; Caspase 9, cysteine-aspartic acid protease 9; PARP, poly(ADP-ribose) polymerase.

Article Snippet: Immortalized human endometrial stromal T-HESCs were obtained from the American Type Culture Collection (#CRL-4003), and immortalized human endometriotic 12Z cells were purchased from Applied Biological Materials (#T0764).

Techniques: